The Proteomics Protocols Handbook

The Proteomics Protocols Handbook

Timely and highly practical, The Proteomics Protocols Handbook offers molecular biologists, protein chemists, clinical and medicinal chemists, biochemists, and microbiologists a comprehensive collection of today's best tools for ...

Author: John M. Walker

Publisher: Springer Science & Business Media

ISBN: 9781592598908

Category: Science

Page: 988

View: 727

Hands-on researchers describe in step-by-step detail 73 proven laboratory methods and bioinformatics tools essential for analysis of the proteome. These cutting-edge techniques address such important tasks as sample preparation, 2D-PAGE, gel staining, mass spectrometry, and post-translational modification. There are also readily reproducible methods for protein expression profiling, identifying protein-protein interactions, and protein chip technology, as well as a range of newly developed methodologies for determining the structure and function of a protein. The bioinformatics tools include those for analyzing 2D-GEL patterns, protein modeling, and protein identification. All laboratory-based protocols follow the successful Methods in Molecular BiologyTM series format, each offering step-by-step laboratory instructions, an introduction outlining the principle behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls.
Categories: Science

The Protein Protocols Handbook

The Protein Protocols Handbook

Since the publication of the bestselling second edition of John Walker’s widely acclaimed Protein Protocols Handbook, there have been continual methodological developments in the field of protein chemistry.

Author: John M. Walker

Publisher: Springer Nature

ISBN: STANFORD:36105215315776

Category: Science

Page: 2034

View: 963

Since the publication of the bestselling second edition of John Walker’s widely acclaimed Protein Protocols Handbook, there have been continual methodological developments in the field of protein chemistry. This greatly enhanced third edition introduces 57 critically important new chapters, as well as significantly updating the previous edition's tried-and-true methods. Although the timely new chapters are spread throughout all of the book, the vital section on post-translational modifications has been expanded most to reflect the increasing importance of these modifications in the understanding of protein function. Each readily reproducible method follows the highly praised format of the Methods in Molecular BiologyTM series, offering a concise summary of its basic theory, a complete materials list, a step-by-step protocol for its successful execution, and extensive notes on avoiding pitfalls, or on modifying the method to function within your own experimental circumstances. The expert authors of each chapter have demonstrated a hands-on mastery of the methods described, fine-tuned here for optimal productivity. Comprehensive, cutting-edge, and highly practical, The Protein Protocols Handbook, Third Edition is today's indispensable benchtop manual and guide, not only for all those new to the protein chemistry laboratory, but also for those established workers seeking to broaden their armamentarium of techniques in the urgent search for rapid and robust results
Categories: Science

Guide to Protein Purification

Guide to Protein Purification

In ''The Proteomics Protocol Handbook'' (J. M. Walker, ed.), pp. 215–222. Humana Press, Totowa, NJ. Rabilloud, T. (1990). Mechanisms of protein silver staining in polyacrylamide gels: A 10-year synthesis. Electrophoresis 11, 785–794.

Author: Richard R Burgess

Publisher: Academic Press

ISBN: 0080923178

Category: Science

Page: 900

View: 116

The 2e of this classic Guide to Protein Purification provides a complete update to existing methods in the field, reflecting the enormous advances made in the last two decades. In particular, proteomics, mass spectrometry, and DNA technology have revolutionized the field since the first edition’s publication but through all of the advancements, the purification of proteins is still an indispensable first step in understanding their function. This volume examines the most reliable, robust methods for researchers in biochemistry, molecular and cell biology, genetics, pharmacology and biotechnology and sets a standard for best practices in the field. It relates how these traditional and new cutting-edge methods connect to the explosive advancements in the field. This "Guide to" gives imminently practical advice to avoid costly mistakes in choosing a method and brings in perspective from the premier researchers while presents a comprehensive overview of the field today. Gathers top global authors from industry, medicine, and research fields across a wide variety of disciplines, including biochemistry, genetics, oncology, pharmacology, dermatology and immunology Assembles chapters on both common and less common relevant techniques Provides robust methods as well as an analysis of the advancements in the field that, for an individual investigator, can be a demanding and time-consuming process
Categories: Science

Analysis of Biological Networks

Analysis of Biological Networks

The Proteomics Protocols Handbook, pp. 629— 652. Humana Press, Totowa, NJ, 2005. 17. 8. Fields and O. K. Song. A novel genetic system to detect protein—protein interactions. Nature, 340:245—246, 1989. 18. T. B. Fischer, M. Paczkowski, ...

Author: Björn H. Junker

Publisher: John Wiley & Sons

ISBN: 9781118209912

Category: Computers

Page: 368

View: 663

An introduction to biological networks and methods for theiranalysis Analysis of Biological Networks is the first book of itskind to provide readers with a comprehensive introduction to thestructural analysis of biological networks at the interface ofbiology and computer science. The book begins with a brief overviewof biological networks and graph theory/graph algorithms and goeson to explore: global network properties, network centralities,network motifs, network clustering, Petri nets, signal transductionand gene regulation networks, protein interaction networks,metabolic networks, phylogenetic networks, ecological networks, andcorrelation networks. Analysis of Biological Networks is a self-containedintroduction to this important research topic, assumes no expertknowledge in computer science or biology, and is accessible toprofessionals and students alike. Each chapter concludes with asummary of main points and with exercises for readers to test theirunderstanding of the material presented. Additionally, an FTP sitewith links to author-provided data for the book is available fordeeper study. This book is suitable as a resource for researchers in computerscience, biology, bioinformatics, advanced biochemistry, and thelife sciences, and also serves as an ideal reference text forgraduate-level courses in bioinformatics and biologicalresearch.
Categories: Computers

Microbial Proteomics Development in Technologies and Applications

Microbial Proteomics  Development in Technologies and Applications

[http://dx.doi.org/10.1016/j.ddtec.2006.09.013] [PMID: 24980539] [6] Toscano M, de Grandi R, Drago L. Proteomics: the new era of microbiology. MicrobiologiaMedica 2017 ... 5432063] [19] Walker JM, Ed. The proteomics protocols handbook.

Author: Divakar Sharma

Publisher: Bentham Science Publishers

ISBN: 9789811491429

Category: Science

Page: 236

View: 447

This volume brings current knowledge of proteomics technologies and related developments with special reference to diseases caused by microbes. The editor has compiled chapters written by expert academicians which distill the information about useful methods in microbial proteomics for the benefit of readers. Chapters cover several methods used to investigate the microbial proteome and special topics such as antimicrobial drug resistance mechanisms, biomarker developments, post translational modifications. Key Features: -overview of several biochemical methods in proteomics -full-color, high quality images of the most frequent technologies and applications -concise, well organized, and didactic format -updates in basic applied information -bibliographic references -information on proteomics for tuberculosis treatment This reference work is intended for researchers seeking information on laboratory techniques applied in proteomics research and microbiology.
Categories: Science

Biomolecular and Bioanalytical Techniques

Biomolecular and Bioanalytical Techniques

In: The Proteomics Protocols Handbook (ed. J.M. Walker). Humana Press. 34 Lakowicz, J.R. (2006). Principles of Fluorescence Spectroscopy. Springer-Verlag. 35 Moon, J.-H., Kim, S.K., Sehlstedt, U. et al. (1996).

Author: Vasudevan Ramesh

Publisher: John Wiley & Sons

ISBN: 9781119483984

Category: Science

Page: 576

View: 796

An essential guide to biomolecular and bioanalytical techniques and their applications Biomolecular and Bioanalytical Techniques offers an introduction to, and a basic understanding of, a wide range of biophysical techniques. The text takes an interdisciplinary approach with contributions from a panel of distinguished experts. With a focus on research, the text comprehensively covers a broad selection of topics drawn from contemporary research in the fields of chemistry and biology. Each of the internationally reputed authors has contributed a single chapter on a specific technique. The chapters cover the specific technique’s background, theory, principles, technique, methodology, protocol and applications. The text explores the use of a variety of analytical tools to characterise biological samples. The contributors explain how to identify and quantify biochemically important molecules, including small molecules as well as biological macromolecules such as enzymes, antibodies, proteins, peptides and nucleic acids. This book is filled with essential knowledge and explores the skills needed to carry out the research and development roles in academic and industrial laboratories. A technique-focused book that bridges the gap between an introductory text and a book on advanced research methods Provides the necessary background and skills needed to advance the research methods Features a structured approach within each chapter Demonstrates an interdisciplinary approach that serves to develop independent thinking Written for students in chemistry, biological, medical, pharmaceutical, forensic and biophysical sciences, Biomolecular and Bioanalytical Techniques is an in-depth review of the most current biomolecular and bioanalytical techniques in the field.
Categories: Science

Biotechnologies for Plant Mutation Breeding

Biotechnologies for Plant Mutation Breeding

Electrophoresis 20:601–605 Giorgianni F, Beranova-Giorgianni S (2005) Analysis of the proteomes in human tissues by in-gel isoelectric focusing and mass spectrometry. In: Walker JM (ed) The proteomic protocols handbook.

Author: Joanna Jankowicz-Cieslak

Publisher: Springer

ISBN: 9783319450216

Category: Science

Page: 340

View: 321

This book is open access under a CC BY-NC 2.5 license. This book offers 19 detailed protocols on the use of induced mutations in crop breeding and functional genomics studies, which cover topics including chemical and physical mutagenesis, phenotypic screening methods, traditional TILLING and TILLING by sequencing, doubled haploidy, targeted genome editing, and low-cost methods for the molecular characterization of mutant plants that are suitable for laboratories in developing countries. The collection of protocols equips users with the techniques they need in order to start a program on mutation breeding or functional genomics using both forward and reverse-genetic approaches. Methods are provided for seed and vegetatively propagated crops (e.g. banana, barley, cassava, jatropha, rice) and can be adapted for use in other species.
Categories: Science

Proteomics of Biological Systems

Proteomics of Biological Systems

Protein Phosphorylation Using Mass Spectrometry Techniques Bryan M. Ham ... Moser, K.;White, F.M. J. Proteome Res. ... Leimgruber, R.M. In The Proteomics Protocols Handbook, Walker, J.M., Ed. Totowa, NJ: Humana Press, 2005; p. 4.

Author: Bryan M. Ham

Publisher: John Wiley & Sons

ISBN: 9781118137031

Category: Science

Page: 376

View: 795

Phosphorylation is the addition of a phosphate (PO4) group to a protein or other organic molecule. Phosphorylation activates or deactivates many protein enzymes, causing or preventing the mechanisms of diseases such as cancer and diabetes. This book shows how to use mass spectrometry to determine whether or not a protein has been correctly modified by the addition of a phosphate group. It also provides a combination of detailed, step-by-step methodology for phosphoproteomic sample preparation, mass spectral instrumental analysis, and data interpretation approaches. Furthermore, it includes the use of bioinformatic Internet tools such as the Blast2GO gene ontology (GO) tool, used to help understand and interpret complex data collected in these studies.
Categories: Science

Proteomics Technologies and Applications

Proteomics Technologies and Applications

Proteomics analysis to assess the role of Mitochondria in BRCA1mediated breast tumorigenesis. Proteome. 2018;6(2):pii: E16. DOI: 10.3390/proteomes6020016 [20] Seillier-Moiseiwitsch F. The Proteomics Protocols Handbook.

Author: Ibrokhim Y. Abdurakhmonov

Publisher: BoD – Books on Demand

ISBN: 9781789846102

Category: Science

Page: 132

View: 736

Proteomics Technologies and Applications reviews and describes the nature and application of molecules with proteins or peptides, and elucidates and predicts the possible molecular and physiological causes related to changing proteomic profiles. Chapters target various methods and tools available for analysis, detection, separation, quantification, and localization of cell proteomes of a biological system, which are helpful as biomarkers for various disease prognoses and diagnoses.
Categories: Science

Computational Methods for Mass Spectrometry Proteomics

Computational Methods for Mass Spectrometry Proteomics

Magrane M, Martin MJ, O'Donovan C and Apweiler R 2005 Protein sequence databases The Proteomics Protocols Handbook. Humana Press. Mann M and Wilm M 1994 Error-tolerant identification of peptides in sequence databases by peptide sequence ...

Author: Ingvar Eidhammer

Publisher: John Wiley & Sons

ISBN: 0470724293

Category: Medical

Page: 296

View: 580

Proteomics is the study of the subsets of proteins present in different parts of an organism and how they change with time and varying conditions. Mass spectrometry is the leading technology used in proteomics, and the field relies heavily on bioinformatics to process and analyze the acquired data. Since recent years have seen tremendous developments in instrumentation and proteomics-related bioinformatics, there is clearly a need for a solid introduction to the crossroads where proteomics and bioinformatics meet. Computational Methods for Mass Spectrometry Proteomics describes the different instruments and methodologies used in proteomics in a unified manner. The authors put an emphasis on the computational methods for the different phases of a proteomics analysis, but the underlying principles in protein chemistry and instrument technology are also described. The book is illustrated by a number of figures and examples, and contains exercises for the reader. Written in an accessible yet rigorous style, it is a valuable reference for both informaticians and biologists. Computational Methods for Mass Spectrometry Proteomics is suited for advanced undergraduate and graduate students of bioinformatics and molecular biology with an interest in proteomics. It also provides a good introduction and reference source for researchers new to proteomics, and for people who come into more peripheral contact with the field.
Categories: Medical