Preparative Layer Chromatography

Preparative Layer Chromatography

Thin-layer chromatography (TLC) especially proved to be very useful for analysis and isolation of small amounts of some compounds. The most significant and advantageous points of the TLC technique are its speed, cheapness, and capacity ...

Author: Teresa Kowalska

Publisher: CRC Press

ISBN: 9781420005820

Category: Science

Page: 424

View: 836

Preparative Layer Chromatography explains how this method is used for separating large quantities of mixtures containing a wide variety of important compounds. It offers a broad review of preparative layer chromatography (PLC) applications and adaptable working procedures for microseparations involving organic, inorganic, and organometallic compounds. The book contains theoretical background, chemical principles, and relevance of preparative layer chromatography (PLC) to a wide range of applications, particularly in the study of pharmaceuticals and biochemistry. Written by many of the best known and most knowledgeable specialists in the field, the chapters describe all the necessary techniques, current procedures, and superior strategies for selecting the most suitable eluents and designing application-specific PLC systems based on the data being sought. They provide comprehensive instructions, surrounding issues, and suggestions for optimizing optional working techniques within the framework of PLC. The book also provides a complete coverage of bulk sorbents and precoated chromatographic plates available on the international market. A comprehensive, yet accessible source of information, Preparative Layer Chromatography is a relevant and practical text for experienced as well as novice researchers and practitioners involved in analytical, environmental, geochemical, biological, medicinal, and pharmaceutical analysis.
Categories: Science

Handbook of Thin Layer Chromatography

Handbook of Thin Layer Chromatography

preparative. layer. chromatography. bility than homemade layers. Three types of precoated preparative plates—silica, alumina, and RP-2—are generally commercially available in layer thicknesses of 0.5–2 mm. It is generally accepted that ...

Author: Joseph Sherma

Publisher: CRC Press

ISBN: 9780824748661

Category: Science

Page: 1356

View: 846

In this third edition, more than 40 renowned authorities introduce and update chapters on the theory, fundamentals, techniques, and instrumentation of thin-layer chromatography (TLC) and high-performance thin-layer chromatography (HPTLC), highlighting the latest procedures and applications of TLC to 19 important compound classes and coverage of TLC applications by compound type. Easily adaptable to industrial scenarios , the Handbook of Thin-Layer Chromatography, Third Edition supports practical research strategies with extensive tables of data, offers numerous figures that illustrate techniques and chromatograms, and includes a glossary as well as a directory of equipment suppliers.
Categories: Science

Recent Advances in Thin Layer Chromatography

Recent Advances in Thin Layer Chromatography

This volume represents the proceedings of a meeting in Brighton in 1987 whieh formed part of a eontinuing series of one and two day events on TLC organized by the Chromatographie Soeiety either alone or, like this one, in eonjunetion with ...

Author: F.A.A. Dallas

Publisher: Springer Science & Business Media

ISBN: 9781489922212

Category: Medical

Page: 247

View: 150

For many years TLC has suffered from the image of being a low sensitivity, low resolution, non-quantitative teehnique, suitable for chemists, but not a tool for real ehromatographers. Whilst perhaps true in the past this attitude no longer ref1eets the eapabi1ities of modern instrumenta1ized TLC in all its many forms. This volume represents the proceedings of a meeting in Brighton in 1987 whieh formed part of a eontinuing series of one and two day events on TLC organized by the Chromatographie Soeiety either alone or, like this one, in eonjunetion with other learned bodies. These meetings are designed to keep ehromatographers up to date with the latest deve10pments and help promote a more positive image of TLC. lan Wilson November 1987 v CONTENTS The Chromatographic Society xi Contemporary Thin-layer Chromatography: An introduction IoD. Wilson INSTRUMENTATION Quantitative Methods in Thin-layer Chromatography C.F. Poole, S.K. Poole and T.A. Dean 11 Fluorescence Line-narrowing Spectroscopy: A New and Highly Selective Detection Technique for Thin-layer and Liquid Chromatography J.W. Hofstraat, C. Gooijer, U.A. Th. Brinkman and N.H. Velthorst 29 Qualitative and Quantitative Image Analysis of Fluorescence from High Performance Thi. n-layer Chromatography R.M. Belchamber, S.J. Brinkworth, H. Read and J.D.M. Roberts 37 Analytical Rotation Planar Chromatography Sz. Nyiredy, K. Dallenbach-Toelke and O. Sticher 45 Analytical and Preparative Overpressured Layer Chromatography E. Mincsovics and E. Tyihak 57 Anticircular Planar Chromatography: Analytical and Preparative Aspects H. Traitler and A.
Categories: Medical

Modern Thin Layer Chromatography

Modern Thin Layer Chromatography

PREPARATIVE THIN - LAYER CHROMATOGRAPHY Preparative thin - layer chromatography ( TLC ) is often an alternative to column chromatography . Layers having a thickness of 2 mm can hold up to 750 mg of sample ( 1 ) .

Author: Nelu Grinberg

Publisher: CRC Press

ISBN: 0824781384

Category: Science

Page: 504

View: 841

Provides chemists with an in-depth account of chromatographic phenomena and a detailed reference guide to the various choices in optimizing chromatographic separations of enantiomers. Clarifies how thin-layer chromatography differs from, but can be used as a pilot procedure for, high-performance liq
Categories: Science

Thin Layer Chromatography Revised And Expanded

Thin Layer Chromatography  Revised And Expanded

B. Forced-Flow Preparative Planar Chromatography Forced-flow planar chromatography (FFPC) with the use of external pressure (overpressured layer chromatography, OPLC) or centrifugal force (rotation planar chromatography, ...

Author: Bernard Fried

Publisher: CRC Press

ISBN: 9780824742089

Category: Science

Page: 512

View: 304

The fourth edition of this work emphasizes the general practices and instrumentation involving TLC and HPTLC, as well as their applications based on compound types, while providing an understanding of the underlying theory necessary for optimizing these techniques. The book details up-to-date qualitative and quantitative densitometric experiments on organic dyes, lipids, antibiotics, pharmaceuticals, organic acids, insecticides, and more.
Categories: Science

Practice of Thin Layer Chromatography

Practice of Thin Layer Chromatography

TABLE 15.1 Commercially Available Preparative Layer Sorbents ( Brinkmann ) Maximum Layer Thickness ( mm ) Sorbent UV Indicator 254 254 and 366 2 2 2 Aluminum oxide 60a PF - 254 PF - 254 + 366 Aluminum oxide 150 PF - 254 PF - 254 + 366 ...

Author: Joseph C. Touchstone

Publisher: John Wiley & Sons

ISBN: 0471612227

Category: Science

Page: 400

View: 839

This Third Edition provides all the basic applications needed to practice thin layer chromatography (TLC). New material includes: the latest techniques on sample preparation and zone detection, the hybridization of TLC with high performance liquid chromatography (HPLC) as it's been developing in the last few years, emphasis on numerous applications of HPTLC involving pharmaceuticals and drugs, plus the fundamental studies of mechanisms, theories and the optimization of TLC.
Categories: Science

Thin Layer Chromatography in Phytochemistry

Thin Layer Chromatography in Phytochemistry

After achieving satisfactory separation, spots of interest were cut into small strips and flavonoids were extracted with a suitable solvent.2 Today, paper is replaced with different sorbents and preparative layer chromatography (PLC) is ...

Author: Monika Waksmundzka-Hajnos

Publisher: CRC Press

ISBN: 1420046780

Category: Science

Page: 896

View: 565

Thin layer chromatography (TLC) is increasingly used in the fields of plant chemistry, biochemistry, and molecular biology. Advantages such as speed, versatility, and low cost make it one of the leading techniques used for locating and analyzing bioactive components in plants. Thin Layer Chromatography in Phytochemistry is the first source devoted to supplying state-of-the-art information on TLC as it applies to the separation, identification, quantification, and isolation of medicinal plant components. Renowned scientists working with laboratories around the world demonstrate the applicability of TLC to a remarkable diversity of fields including plant genetics, drug discovery, nutraceuticals, and toxicology. Elucidates the role of plant materials in the pharmaceutical industry... Part I provides a practical review of techniques, relevant materials, and the particular demands for using TLC in phytochemical applications. The text explains how to determine the biological activity of metabolites and assess the effectiveness of herbal medicines and nutritional supplements. Part II concentrates on TLC methods used to analyze specific plant-based metabolite classes such as carbohydrates, proteins, alkaloids, flavonoids, terpenes, etc. Organized by compound type, each chapter discusses key topics such as sample preparation, plate development, zone detection, densitometry, and biodetection. Demonstrates practical methods that can be applied to a wide range of disciplines... From identification to commercial scale production and quality control, Thin Layer Chromatography in Phytochemistry is an essential bench-top companion and reference on using TLC for the study of plant-based bioactive compounds.
Categories: Science

Thin Layer Chromatography in Drug Analysis

Thin Layer Chromatography in Drug Analysis

3.8 prepArAtive lAyer ChrOmAtOgrAphy Although Preparative Layer Chromatography (PLC) might not often be used during drug analysis, there may come a time when an unknown zone appears in a sample chromatogram, either arising from a new ...

Author: Lukasz Komsta

Publisher: CRC Press

ISBN: 9781466507159

Category: Medical

Page: 1067

View: 462

Used routinely in drug control laboratories, forensic laboratories, and as a research tool, thin layer chromatography (TLC) plays an important role in pharmaceutical drug analyses. It requires less complicated or expensive equipment than other techniques, and has the ability to be performed under field conditions. Filling the need for an up-to-date, complete reference, Thin Layer Chromatography in Drug Analysis covers the most important methods in pharmaceutical applications of TLC, namely, analysis of bulk drug material and pharmaceutical formulations, degradation studies, analysis of biological samples, optimization of the separation of drug classes, and lipophilicity estimation. The book is divided into two parts. Part I is devoted to general topics related to TLC in the context of drug analysis, including the chemical basis of TLC, sample pleparation, the optimization of layers and mobile phases, detection and quantification, analysis of ionic compounds, and separation and analysis of chiral substances. The text addresses the newest advances in TLC instrumentation, two-dimensional TLC, quantification by slit scanning densitometry and image analysis, statistical processing of data, and various detection and identification methods. It also describes the use of TLC for solving a key issue in the drug market—the presence of substandard and counterfeit pharmaceutical products. Part II provides an in-depth overview of a wide range of TLC applications for separation and analysis of particular drug groups. Each chapter contains an introduction about the structures and medicinal actions of the described substances and a literature review of their TLC analysis. A useful resource for chromatographers, pharmacists, analytical chemists, students, and R&D, clinical, and forensic laboratories, this book can be utilized as a manual, reference, and teaching source.
Categories: Medical

Forced Flow Layer Chromatography

Forced Flow Layer Chromatography

Therefore, it is a pleasant circumstance and fact that practically all forced-flow layer liquid chromatographic techniques can be used for preparative separation. Rotation planar chromatography (RPC, named also as centrifugal layer ...

Author: Erno Tyihak

Publisher: Elsevier

ISBN: 9780124202122

Category: Science

Page: 524

View: 248

Forced-Flow Layer Chromatography takes a close look at the specifics of forced-flow layer chromatography techniques, from their evolution to the nuances of using these techniques in a variety of applications where traditional thin-layer chromatography (TLC) and high-performance thin-layer chromatography (HPTLC) are not as effective. This book presents a number of variations of TLC techniques, with special emphasis on the overpressured-layer chromatography (OPLC) technique and newer developments such as the BioArena System for biomedical analysis. The versatility of these forced-flow techniques opens up new avenues for the analysis of a large number of samples for high-throughput screening and for the analysis of very complex matrices, while the development of BioArena extends the use of these techniques to challenging new areas of bioanalysis. Details a variety of forced-flow techniques, explaining how they markedly reduce developing time and result in less lateral diffusion and more compact spots Emphasizes the benefits of OPLC separation techniques, a method pioneered by the authors nearly forty years ago Discusses new developments, such as the BioArena system used to facilitate detection, isolation, and identification of new antimicrobials, antineoplastics, biopesticides, and other biologically active substances
Categories: Science