Microbial Proteomics Development in Technologies and Applications

Microbial Proteomics  Development in Technologies and Applications

Along with these applications, microbial proteomics is also providing support in the elucidation of bacterial metabolic pathways and the relationship between the microorganisms and human health. The microbial proteomics has seen ...

Author: Divakar Sharma

Publisher: Bentham Science Publishers

ISBN: 9789811491429

Category: Science

Page: 236

View: 263

This volume brings current knowledge of proteomics technologies and related developments with special reference to diseases caused by microbes. The editor has compiled chapters written by expert academicians which distill the information about useful methods in microbial proteomics for the benefit of readers. Chapters cover several methods used to investigate the microbial proteome and special topics such as antimicrobial drug resistance mechanisms, biomarker developments, post translational modifications. Key Features: -overview of several biochemical methods in proteomics -full-color, high quality images of the most frequent technologies and applications -concise, well organized, and didactic format -updates in basic applied information -bibliographic references -information on proteomics for tuberculosis treatment This reference work is intended for researchers seeking information on laboratory techniques applied in proteomics research and microbiology.
Categories: Science

Microbial Proteomics

Microbial Proteomics

Immune response Vaccine development Bacterium–host interaction Bacterium (extracellular) Epidemiology ... The diagram illustrates the diverse applications of proteomic technologies that have been used to characterize bacterial pathogens ...

Author: Ian Humphery-Smith

Publisher: John Wiley & Sons

ISBN: 9780471973157

Category: Science

Page: 530

View: 903

Discover important lessons learned about whole organism biologyvia microbial proteomics This text provides an exhaustive analysis and presentation ofcurrent research in the field of microbial proteomics, with anemphasis on new developments and applications and future directionsin research. The editors and authors show how and why the relativesimplicity of microbes has made them attractive targets forextensive experimental manipulation in a quest for both improveddisease prevention and treatment and an improved understanding ofwhole organism functional biology. In particular, the textdemonstrates how microbial proteomic analyses can aid in drugdiscovery, including identification of new targets, noveldiagnostic markers, and lead optimization. Each chapter is written by one or more leading experts in thefield and carefully edited to ensure a consistent and thoroughapproach throughout. Methods, technologies, and tools associatedwith the most promising approaches are stressed. Key topics coveredinclude: Microbial pathogenesis at the proteome level Whole cell modeling Structural proteomics and computational analysis Biomolecular interactions Physiological proteomics Metabolic reconstruction using proteomics data While presenting the practical utility of proteomics data, thetext is also clear on the field's current limitations, pointing toareas where further investigation is needed. Offering a state-of-the-art perspective from internationallyrecognized experts, this text is ideally suited for researchers andstudents across the gamut of genomic sciences, includingbiochemistry, microbiology, molecular biology, genetics, biomedicaland pharmaceutical sciences, biotechnology, and veterinaryscience.
Categories: Science

Applications of Genomics and Proteomics for Analysis of Bacterial Biological Warfare Agents

Applications of Genomics and Proteomics for Analysis of Bacterial Biological Warfare Agents

of Bacterial Biological Warfare Agents V.G. DelVecchio and V. Krcmery ( Eds . ) IOS Press , 2003 Proteome of ... Here we demonstrate the proteome technology as a tool for creation of Francisella tularensis protein database and its ...

Author: Vito G. DelVecchio

Publisher: IOS Press

ISBN: 1586033433

Category: Bacterial diseases

Page: 260

View: 494

Categories: Bacterial diseases

Sequencing Technologies in Microbial Food Safety and Quality

Sequencing Technologies in Microbial Food Safety and Quality

6.4.7 Applications in Fermented Foods 6.4.8 Application for Identification of Food Allergen 6.4.9 Application for Food ... Proteomics applies in different fields such as drug development, plant breeding, characterization of microbes, ...

Author: Devarajan Thangardurai

Publisher: CRC Press

ISBN: 9781000376395

Category: Technology & Engineering

Page: 500

View: 869

Molecular landscape for food safety analysis is rapidly revolutionizing because of high resolution and value added resulting analysis of next-generation sequencing (NGS) approaches. These modern sequencing technologies drive worldwide advancements in food safety and quality. Sequencing Technologies in Microbial Food Safety and Quality reviews several practices in that NGS contributes to foodborne pathogens functional characterization, management and control. This book focuses on potential uses of sequencing technologies in microbial food safety and quality and highlights present challenges in the food industry. Key Features: Application of whole genome sequencing technologies in disease diagnostics, surveillance, transmission, and outbreak investigation in food sector Impact of sequencing tools in the area of food microbiology Recent advances in genomic DNA sequencing of microbial species from single cells Microbial bioinformatics resources for food microbiology High-throughput insertion tracking by deep sequencing for the analysis of food pathogens This book includes contributions from experts who have manipulated sequencing tools in relation to microbial food safety and quality. Presenting comprehensive details about NGS approaches in food science, this book is an updated and reliable reference for food scientists, nutritionists, food product investigators to study and implement the sequencing technologies for developing quality and safe food. This book would also serve as informative resource for food industry officials, government researchers, food science or food nutrition students who seek comprehensive knowledge about the role of emerging sequencing technologies in revolutionizing the food industry.
Categories: Technology & Engineering

Mass Spectrometry for Microbial Proteomics

Mass Spectrometry for Microbial Proteomics

However, current development in systems biology will eventually provide a better understanding of the immune system ... Historically, the portfolio of technologies that are now generally referred to as proteomics has been studied mainly ...

Author: Haroun N. Shah

Publisher: John Wiley & Sons

ISBN: 1119991927

Category: Science

Page: 534

View: 368

New advances in proteomics, driven largely by developments in mass spectrometry, continue to reveal the complexity and diversity of pathogenic mechanisms among microbes that underpin infectious diseases. Therefore a new era in medical microbiology is demanding a rapid transition from current procedures to high throughput analytical systems for the diagnosis of microbial pathogens. This book covers the broad microbiological applications of proteomics and mass spectrometry. It is divided into six sections that follow the general progression in which most microbiology laboratories are approaching the subject –Transition, Tools, Preparation, Profiling by Patterns, Target Proteins, and Data Analysis.
Categories: Science

Proteomics of Microbial Human Pathogens

Proteomics of Microbial Human Pathogens

Proteomic techniques are continuously being developed and there is now a great diversity of methods and ... obtained recently through proteomic technology should be useful for developing new diagnostic and therapeutic applications for ...

Author: Nelson C. Soares

Publisher: Frontiers Media SA

ISBN: 9782889450886

Category: Electronic book

Page: 154

View: 946

According to the World Health Organization (WHO), in 2012 infectious diseases and related conditions account for more than 70% of premature deaths across 22 African countries and estimated 450 000 people worldwide developed multi-drug resistant tuberculosis. This alarming situation, of great public health concern, calls for the urgent development of novel and efficient responding strategies. The employment of important research platforms, such as genomics and proteomics, has contributed significant insight into the mechanisms underlying microbial infection and microbe-host interaction. In this Frontiers Research Topic, we aim to produce a timely and pertinent discussion regarding the current status of “Proteomics of microbial Human pathogens” and the role of proteomics in combating the challenges posed by microbial infection and indeed acquired anti-microbial resistance. As the field of proteomics progressed from 2-DE gel based approaches to modern LC-MS/MS based workflows, remarkable advances have been reported in terms of data quantity and quality. Given the immediate and enormous advantages that high resolution and accurate mass spectrometers have brought to the field, proteomics has now evolved into a robust platform capable of generating large amounts of comprehensive data comparable to that reported previously in genomics studies. For example, detection of the complete yeast proteome has been reported and other small proteomes, such as those of bacteria, are within reach. Mass spectrometry-based proteomics has become an essential tool for biologists and biochemists, and is now considered by many as an essential component of modern structural biology. Additionally, the introduction of high-resolution mass spectrometers has driven the development of various different strategies aimed at accurate quantification of absolute and relative amount of protein(s) of interest. Emerging targeted mass spectrometry methodologies such as; Selected Reaction Monitoring (SRM), Parallel Reaction Monitoring (PRM) and SWATH, are perhaps the latest breakthrough within the proteomics community. Indeed, through a label free approach, targeted mass spectrometry offers an unequalled capability to characterize and quantify a specific set of proteins reproducibility, in any biological sample. Usefully, Aebersold and colleagues have recently generated and validated a number of assays to quantify 97% of the 4,012 annotated Mycobacterium tuberculosis (Mtb) proteins by SRM. As such, the Mtb Proteome library represents a valuable experimental resource that will undoubtedly bring new insight to the complex life cycle of Mtb. Finally, as reviewed recently in Frontiers Research Topic, mass spectrometry-based proteomics has had a tremendous impact on our current understanding of post translational modification (PTM) in bacteria including the key role of PTMs during interaction of pathogenic bacteria and host interactions. We believe that our understanding of microbial Human pathogens has benefited enormously from both 2-DE gel and modern LC-MS/MS based proteomics. It is our wish to produce an integrated discussion surrounding this topic to highlight the existing synergy between these research fields. We envisage this Research Topic as a window to expert opinions and perspectives on the realistic practicalities of proteomics as an important tool to address healthcare problems caused by microbial pathogens.
Categories: Electronic book

Advances in Probiotic Technology

Advances in Probiotic Technology

However, since the beginning of the proteomic era, the development and application of proteomics strictly relied on the capability of analytical resources and technologies to perform high-throughput proteomic studies.

Author: Petra ger

Publisher: CRC Press

ISBN: 9781498734585

Category: Science

Page: 384

View: 577

The future prospects of probiotics lie in the successful application of individual strains with specific beneficial effects on the host. This development implies that not only the most robust strains are selected but also strains with a promising probiotic function with moderate or high sensitivity to processing stresses. This also means an increasing variety of probiotic strains with different functions. Therefore the processing of probiotics becomes an important issue. The strains have to be cultivable and proper growth conditions have to be known. Another very important step in processing is the preservation step. This includes either the freezing and frozen storage or the drying and storage in powder form. The fermentation, drying, and storage processes are highly interrelated. Therefore a holistic approach has to be chosen for the production of highly effective probiotic formulation. The book comprises state-of-the-art knowledge on isolation and characterization of probiotics as well as processing (fermentation, freezing, drying, and storage) and application of probiotics in different food products. This book will serve as a guidebook to researchers, technologists, and industry professionals in the field of probiotics.
Categories: Science

Microbial Biotechnology

Microbial Biotechnology

Genomics and Proteomics of Microbial Pathogenesis The official announcement on 26 June 2000 of the completion of the ... Scientists are exploiting and developing technologies that encompass or overlap the triad of genomics, proteomics ...

Author: Yuan Kun Lee

Publisher: World Scientific Publishing Company

ISBN: 9789814513098

Category: Science

Page: 896

View: 312

The rapidly expanding molecular biological techniques and approaches have significant impact on microbial biotechnology, hence the need for the addition of four new chapters in the third edition of this textbook — “Chapter 3: Application of ‘Omics’ Technologies in Microbial Fermentation”, “Chapter 5: Microbial Genome Mining for Identifying Antimicrobial Targets”, “Chapter 21: Bacterial Biofilm: Molecular Characterization and Impacts on Water Management” and “Chapter 23: Microbial Biomining”. “Chapter 15: Transgenic Plants” has been completely revised while most of the other chapters have been thoroughly updated in this new edition. There already exist a number of excellent general textbooks on microbiology and biotechnology that deal with the basic principles of microbial biotechnology. To complement them, this book focuses on the various applications of microbial-biotechnological principles. A teaching-based format is adopted, whereby working problems, as well as answers to frequently asked questions, supplement the main text. The book also includes real life examples of how the application of microbial-biotechnological principles has achieved breakthroughs in both research and industrial production. Although written for polytechnic students and undergraduates, the book contains sufficient information to be used as a reference for postgraduate students and lecturers. It may also serve as a resource book for corporate planners, managers and applied research personnel.
Categories: Science

Fundamental Bacterial Genetics

Fundamental Bacterial Genetics

The fields of bioinformatics and proteomics apply state-of-the-art technology to fundamental problems in biology. Bioinformatics takes the information we have learned through experimentation and uses it to predict characteristics of ...

Author: Nancy Trun

Publisher: John Wiley & Sons

ISBN: 9781444311556

Category: Science

Page: 304

View: 309

Fundamental Bacterial Genetics presents a conciseintroduction to microbial genetics. The text focuses on onebacterial species, Escherichia coli, but draws examples fromother microbial systems at appropriate points to support thefundamental concepts of molecular genetics. A solid balance ofconcepts, techniques and applications makes this book anaccessible, essential introduction to the theory and practice offundamental microbial genetics. FYI boxes - feature key experiments that lead to what we nowknow, biographies of key scientists, comparisons with other speciesand more. Study questions - at the end of each chapter, review and teststudents' knowledge of key chapter concepts. Key references - included both at chapter end and in a fullreference list at the end of the book. Full Chapter on Genomics, Bioinformatics and Proteomics -includes coverage of functional genomics and microarrays. Dedicated website – animations, study resources, webresearch questions and illustrations downloadable for powerpointfiles provide students and instructors with an enhanced,interactive experience.
Categories: Science

Microbial Genomics and Drug Discovery

Microbial Genomics and Drug Discovery

Protein microarray technology recently developed by several labs utilizes protein microchannel microarrays to characterize antibody profiles ... What is the application of proteomics to disease and , in particular , infectious disease ?

Author: Thomas J. Dougherty

Publisher: CRC Press

ISBN: 0203911466

Category: Medical

Page: 272

View: 500

Exploring innovative routes of drug discovery in the postgenomic era, Microbial Genomics and Drug Discovery examines bioinformatic and genomic approaches for the identification, detection, selection, and validation of new antibacterial targets and vaccine candidates. The book discusses potential pathways for effective infection control, inhibition of antibacterial resistance, and acceleration of the drug discovery process. This comprehensive reference describes procedures to recognize common targets in various bacterial species for the design of broad-spectrum antibiotics and clearly illustrates the use of pathway tools in a genomics-based drug discovery project.
Categories: Medical