Biomolecular and Bioanalytical Techniques

Biomolecular and Bioanalytical Techniques

Proteins, nucleic acids (DNA and RNA) and other biomolecules do not workin isolation. ... of binding requires separation of bound and free Biomolecular and Bioanalytical Techniques: Theory, Methodology and Applications, First Edition.

Author: Vasudevan Ramesh

Publisher: John Wiley & Sons

ISBN: 9781119483960

Category: Science

Page: 576

View: 678

An essential guide to biomolecular and bioanalytical techniques and their applications Biomolecular and Bioanalytical Techniques offers an introduction to, and a basic understanding of, a wide range of biophysical techniques. The text takes an interdisciplinary approach with contributions from a panel of distinguished experts. With a focus on research, the text comprehensively covers a broad selection of topics drawn from contemporary research in the fields of chemistry and biology. Each of the internationally reputed authors has contributed a single chapter on a specific technique. The chapters cover the specific technique’s background, theory, principles, technique, methodology, protocol and applications. The text explores the use of a variety of analytical tools to characterise biological samples. The contributors explain how to identify and quantify biochemically important molecules, including small molecules as well as biological macromolecules such as enzymes, antibodies, proteins, peptides and nucleic acids. This book is filled with essential knowledge and explores the skills needed to carry out the research and development roles in academic and industrial laboratories. A technique-focused book that bridges the gap between an introductory text and a book on advanced research methods Provides the necessary background and skills needed to advance the research methods Features a structured approach within each chapter Demonstrates an interdisciplinary approach that serves to develop independent thinking Written for students in chemistry, biological, medical, pharmaceutical, forensic and biophysical sciences, Biomolecular and Bioanalytical Techniques is an in-depth review of the most current biomolecular and bioanalytical techniques in the field.
Categories: Science

Bioanalytical Chemistry

Bioanalytical Chemistry

The current book presents the major experimental techniques for the determination and quantitative analysis of biological macromolecules.

Author: Paolo Ugo

Publisher: de Gruyter

ISBN: 3110589095

Category: Science

Page: 310

View: 399

Bioanalytical chemistry is a rapidly evolving research field due to an increasing focus on the analysis of biological molecules in academia and industry. The current book presents the major experimental techniques for the determination and quantitative analysis of biological macromolecules. In addition, the author introduces the newest approaches in bioanalysis, such as biological sensors and nanostructured analytical devices.
Categories: Science

Advances in Physical Organic Chemistry

Advances in Physical Organic Chemistry

Reif M, Zacharias M. Computer modelling and molecular dynamics simulation of biomolecules. In: Ramesh V, ed. Biomolecular and Bioanalytical Techniques: Theory, Methodology and Applications. Hoboken, NJ: Wiley; 2019. 2.

Author:

Publisher: Academic Press

ISBN: 9780128218211

Category: Science

Page: 192

View: 233

Advances in Physical Organic Chemistry, Volume 54, presents the latest reviews of recent work in physical organic chemistry. The book provides a valuable source of information that is ideal not only for physical organic chemists applying their expertise to both novel and traditional problems, but also for non-specialists across diverse areas who identify a physical organic component in their approach to research. Its hallmark is a quantitative, molecular level understanding of phenomena across a diverse range of disciplines. Reviews the application of quantitative and mathematical methods to help readers understand chemical problems Provides the chemical community with authoritative and critical assessments of the many aspects of physical organic chemistry Covers organic, organometallic, bioorganic, enzymes and materials topics Presents the only regularly published resource for reviews in physical organic chemistry Written by authoritative experts who cover a wide range of topics that require a quantitative, molecular-level understanding of phenomena across a diverse range of disciplines
Categories: Science

Nanobiosensors for Biomolecular Targeting

Nanobiosensors for Biomolecular Targeting

Various materials, technologies, and methods involved in the process of functionalization of biosensing surfaces are ... It leads to utilization of nanomaterials in bioanalytical methods for effective capturing of biomolecules.

Author: Subash C.B. Gopinath

Publisher: Elsevier

ISBN: 9780128139011

Category: Technology & Engineering

Page: 352

View: 354

Nanobiosensors for Bio-molecular Targeting presents the latest analytical methods for the detection of different substances in the range of small molecules to whole cells, exploring the advantages and disadvantages of each method. Biosensors combine the component of biological origin and physicochemical detector to show the presence of analytes in a given sample. The use of bionanotechnology has led to a significant advancement in the progression of nanobiosensors and has been effectively used for biomedical diagnosis. Explains the detection techniques used by nanosensors, exploring the strengths and weaknesses of each for the detection of disease Shows how biosensors are used to detect various types of biomolecules Demonstrates how the use of nanomaterials makes biosensors both cheaper and more efficient
Categories: Technology & Engineering

Bioanalytical Chemistry

Bioanalytical Chemistry

At exactly this time , it seems imperative to provide a small introductory textbook covering the most frequently used instrumental methods of analytical chemistry in molecular biology . The increasingly interdisciplinary nature of ...

Author: Andreas Manz

Publisher: Imperial College Press

ISBN: 1860943713

Category: Science

Page: 206

View: 338

Interdisciplinary knowledge is becoming more and more important to the modern scientist. This invaluable textbook covers bioanalytical chemistry (mainly the analysis of proteins and DNA) and explains everything for the nonbiologist. Electrophoresis, mass spectrometry, biosensors, bioassays, DNA and protein sequencing are not necessarily all included in conventional analytical chemistry textbooks. The book describes the basic principles and the applications of instrumental and molecular methods. It is particularly useful to chemistry and engineering students who already have some basic knowledge about analytical chemistry.
Categories: Science

Analyzing Biomolecular Interactions by Mass Spectrometry

Analyzing Biomolecular Interactions by Mass Spectrometry

Part I Direct MS Based Affinity Techniques Analyzing Biomolecular Interactions ... T.J. (2012) High-field asymmetric waveform ion mobility spectrometry with solvent vapor addition: a potential greener bioanalytical technique.

Author: Jeroen Kool

Publisher: John Wiley & Sons

ISBN: 9783527673421

Category: Science

Page: 400

View: 929

This monograph reviews all relevant technologies based on mass spectrometry that are used to study or screen biological interactions in general. Arranged in three parts, the text begins by reviewing techniques nowadays almost considered classical, such as affinity chromatography and ultrafiltration, as well as the latest techniques. The second part focusses on all MS-based methods for the study of interactions of proteins with all classes of biomolecules. Besides pull down-based approaches, this section also emphasizes the use of ion mobility MS, capture-compound approaches, chemical proteomics and interactomics. The third and final part discusses other important technologies frequently employed in interaction studies, such as biosensors and microarrays. For pharmaceutical, analytical, protein, environmental and biochemists, as well as those working in pharmaceutical and analytical laboratories.
Categories: Science

Practical Skills in Biomolecular Sciences

Practical Skills in Biomolecular Sciences

Sources for further study Center for Drug Evaluation and Research Guidance for Industry Bioanalytical Method Validation . Available : http://www.fda.gov/CDER/GUIDANCE/4252fnl.htm Last accessed : 01/04/07 . [ US FDA guidance / details of ...

Author: Robert H. Reed

Publisher: Pearson Education

ISBN: 0132391155

Category: Science

Page: 574

View: 541

Practical Skills in Biomolecular Science, is an indispensable book for undergraduate students in the life sciences. The book provides useful support at all stages of a degree course and underpins any practical course in biochemistry, biomedical science, genetics, immunology and microbiology. It is also a valuable resource for teachers of biology in colleges and secondary schools. Laboratory and field studies are essential components of undergraduate training in biomolecular science. Practical work must be fully understood and effectively presented, but many students under-perform because they lack basic laboratory skills. This book, now in its third edition, continues to provide students with easy-to-use guidance for laboratory and field studies, but in addition it now covers broader transferable skills. As a result the new edition provides guidance and support over the entire range of a typical undergraduate course in biochemistry and biomedical science.
Categories: Science

New Methods for the Study of Biomolecular Complexes

New Methods for the Study of Biomolecular Complexes

The combination of routine mass analysis with traditionally employed bioanalytical and preparatory methods affords new and powerful approaches for biomolecular study. Presented here have been ideas on the sensible combination of ...

Author: W. Ens

Publisher: Springer Science & Business Media

ISBN: 9789401590464

Category: Science

Page: 354

View: 653

A NATO Advanced Research Workshop entitled New Methods for the Study of Molecular Aggregates was held at Tbe Lodge at Kananaskis Village, Alberta, Canada from 16 -20 June 1996. In fact the meeting was entirely concerned with the problem of analyzing biomolecular complexes, so the title of these proceedings has been altered to give a more precise description of the content. Tbe workshop was hosted by the time-of-flight group of the Department of Physics at the University of Manitoba, and was attended by 64 participants from around the world. '!\venty-one invited talks were given and 27 papers were presented as posters. Of the 48 contributions, 22 papers (12 orals, 10 posters) are included in these proceedings. Tbe subject of the conference was the investigation of noncovalent biomolecular complexes, with particular focus on the application of mass spectrometry to their characterization. '!\vo new ionization techniques introduced in the late 1980s, electrospray ionization (ES I) and matrix-assisted laser desorptionlionization (MALDI), resulted in a breakthrough in mass spectrometry, enabling its use in molecular weight and primary structure determination of biopolymers larger than 100 kDa. Recently it has been discovered that ESI mass spectrometry mayaiso be used to characterize complexes containing noncovalent interactions, thus opening new perspectives for supramolecular chemistry. ESI mass spectrometry has the advantage that the sampie is introduced from a homogenous solution which can be maintained at near physiological conditions of pR, concentration, and temperature.
Categories: Science

Cellular and Biomolecular Recognition

Cellular and Biomolecular Recognition

Understanding interactions among biological molecules and between biomolecules and cells is at the core of diverse and ... KGaA, Weinheim ISBN: 978-3-527-32265-7 XIII Advanced bioanalytical techniques play a significant role in ...

Author: Raz Jelinek

Publisher: John Wiley & Sons

ISBN: 9783527627028

Category: Science

Page: 368

View: 640

With its exploration of the scientific and technological characteristics of systems exploiting molecular recognition between synthetic materials, such as polymers and nanoparticles, and biological entities, this is a truly multidisciplinary book bridging chemistry, life sciences, pharmacology and medicine. The authors introduce innovative biomimetic chemical assemblies which constitute platforms for recruitment of cellular components or biological molecules, while also focusing on physical, chemical, and biological aspects of biomolecular recognition. The diverse applications covered include biosensors, cell adhesion, synthetic receptors, cell patterning, bioactive nanoparticles, and drug design.
Categories: Science

Laser Photodissociation and Spectroscopy of Mass separated Biomolecular Ions

Laser Photodissociation and Spectroscopy of Mass separated Biomolecular Ions

Lasers are also routinely employed to ionize biomolecules, for instance in matrix-assisted laser desorption/ionization ... biophysical and bioanalytical techniques have so far been limited to a handful of laboratories around the world.

Author: Nicolas C. Polfer

Publisher: Springer Science & Business Media

ISBN: 9783319012520

Category: Science

Page: 119

View: 450

This lecture notes book presents how enhanced structural information of biomolecular ions can be obtained from interaction with photons of specific frequency - laser light. The methods described in the book "Laser photodissociation and spectroscopy of mass-separated biomolecular ions" make use of the fact that the discrete energy and fast time scale of photoexcitation can provide more control in ion activation. This activation is the crucial process producing structure-informative product ions that cannot be generated with more conventional heating methods, such as collisional activation. The book describes how the powerful separation capabilities and sensitivity of mass spectrometry (MS) can be combined with the structural insights from spectroscopy by measuring vibrational and electronic spectra of trapped analytes. The implementation of laser-based photodissociation techniques in MS requires basic knowledge of tunable light sources and ion trapping devices. This book introduces the reader to key concepts and approaches in molecular spectroscopy, and the light sources and ion traps employed in such experiments. The power of the methods is demonstrated by spectroscopic interrogation of a range of important biomolecular systems, including peptides, proteins, and saccharides, with laser light in the ultraviolet-visible, and infrared range. The book "Laser photodissociation and spectroscopy of mass-separated biomolecular ions" is an indispensable resource for students and researchers engaged or interested in this emerging field. It provides the solid background of key concepts and technologies for the measurements, discusses state-of-the-art experiments, and provides an outlook on future developments and applications.
Categories: Science